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Pathology and IHC of the pulmonary lymphoepithelioma-like carcinoma. (A and B) Histological analysis. (A) heterologous cells were arranged in clusters (magnification, ×40); (B) the nuclei of heterologous cells were vacuolated and the nucleoli were clear (magnification, ×200; H&E staining); the arrows point at heterologous cells. (C-I) IHC analysis. (C) Cytoplasmic CK5/6 was positive, the arrows point at positive CK5/6 cytoplasm; (D) nuclear P40 was positive; the arrows point at positive P40 nuclear; (E) thyroid transcription factor-1 was negative; (F) Napsin A was negative; (G) nuclear EBER was positive; the arrows point at positive EBER nuclear; (H) proliferation index Ki-67 positive ~50%, the arrows point at positive Ki-67 nuclear; (I) PD-L1 detection microscopic image: Tumor proportion score=55% (magnification, ×100), the arrows point at positive PD-L1 nuclear. CK, cytokeratin; PD-L1, programmed cell death ligand 1; EBER, Epstein-Barr virus-encoded small RNA; IHC, immunohistochemistry.

Journal: Oncology Letters

Article Title: Adjuvant immunotherapy plus chemotherapy and maintenance immunotherapy for pulmonary lymphoepithelioma-like carcinoma with hepatitis B virus infection, KRAS mutation and high expression of programmed death ligand 1: A case report

doi: 10.3892/ol.2026.15612

Figure Lengend Snippet: Pathology and IHC of the pulmonary lymphoepithelioma-like carcinoma. (A and B) Histological analysis. (A) heterologous cells were arranged in clusters (magnification, ×40); (B) the nuclei of heterologous cells were vacuolated and the nucleoli were clear (magnification, ×200; H&E staining); the arrows point at heterologous cells. (C-I) IHC analysis. (C) Cytoplasmic CK5/6 was positive, the arrows point at positive CK5/6 cytoplasm; (D) nuclear P40 was positive; the arrows point at positive P40 nuclear; (E) thyroid transcription factor-1 was negative; (F) Napsin A was negative; (G) nuclear EBER was positive; the arrows point at positive EBER nuclear; (H) proliferation index Ki-67 positive ~50%, the arrows point at positive Ki-67 nuclear; (I) PD-L1 detection microscopic image: Tumor proportion score=55% (magnification, ×100), the arrows point at positive PD-L1 nuclear. CK, cytokeratin; PD-L1, programmed cell death ligand 1; EBER, Epstein-Barr virus-encoded small RNA; IHC, immunohistochemistry.

Article Snippet: Hybridization in situ : EBER monoclonal antibody (cat. no. 25080502; dilution, ready-to-use; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

Techniques: Staining, Virus, Immunohistochemistry

Suppression ability of K-MBR-generated Tregs is preserved (A) Surface expression of activation marker CD69 assessing the ability of CAR-Tregs to respond to target antigen ( n = 2 devices). Suppression assay with cells from 2 healthy donors ( n = 6 devices) in independent experiments to assess. (B) CAR-Tregs suppress T cell proliferation in response to the target antigen in a 24-well plate. (C) Impact of K-MBR culture on CAR-Treg suppressive capacity in the presence of either HLA-A2 + B-LCLs or HLA-A2 - B-LCLs. (D) Representative flow cytometry plots of proliferating Teffs following a 5-day suppression assay. Statistical significance was determined by mixed-model two-way ANOVA for suppression assays; for CD69 expression analysis, t-tests with Šídák correction for multiple comparisons; error bars representing mean ± SEM. ∗: p ≤ 0.05 and ∗∗∗∗: p ≤ 0.0001.

Journal: iScience

Article Title: Perfusion microbioreactor for CAR-Treg manufacturing

doi: 10.1016/j.isci.2026.115246

Figure Lengend Snippet: Suppression ability of K-MBR-generated Tregs is preserved (A) Surface expression of activation marker CD69 assessing the ability of CAR-Tregs to respond to target antigen ( n = 2 devices). Suppression assay with cells from 2 healthy donors ( n = 6 devices) in independent experiments to assess. (B) CAR-Tregs suppress T cell proliferation in response to the target antigen in a 24-well plate. (C) Impact of K-MBR culture on CAR-Treg suppressive capacity in the presence of either HLA-A2 + B-LCLs or HLA-A2 - B-LCLs. (D) Representative flow cytometry plots of proliferating Teffs following a 5-day suppression assay. Statistical significance was determined by mixed-model two-way ANOVA for suppression assays; for CD69 expression analysis, t-tests with Šídák correction for multiple comparisons; error bars representing mean ± SEM. ∗: p ≤ 0.05 and ∗∗∗∗: p ≤ 0.0001.

Article Snippet: The Epstein-Barr Virus (EBV) transformed cell lines SPO B-LCLs (HLA-A2 + ) and BM21 B-LCLs (HLA-A2 - ) (Merck) are part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC) and were obtained from Boardman et al.

Techniques: Generated, Expressing, Activation Assay, Marker, Suppression Assay, Flow Cytometry